EBL AM-50 Protein A, G, and A/G are mediums developed with genetically modified proteins with high alkali-tolerance, which capture monoclonal antibodies for large-scale volumes with chromatography. The resins feature a variety that provides the most versatile combination of chromatographic features for rich yields and high-purity purification of whole IgG for mammalian serum samples.
Key Features:
- The ligands with high alkali-tolerant (up to 0.1-0.5 NaOH) allow for cleaning-in-place (CIP).
- Improved product quality and economy with Up to 100 cycles of lifetime.
- High DBC reduces processing period and medium usage, with all the solutions and consumables saved undoubtedly.
- High-flow and high performance allows for rapid processing procedure.
- Suits for the range form small scale to industry-scale production.
- The ligands are recombinantly produced in Escherichia coli without animal material used in the whole process.
- The mono-dispersed polymer matrix with 50μm size features this product high flow rate, high performance and high DBC.
Specifications:
| Particle Size (μm) | 50 ± 5 % |
| Bead Matrix | Highly cross‐linked polyacrylate polymer |
| Ligand Coupling Method | Epoxy |
| Ligand | highly Alkali-tolerant IG binding protein in E. coli. |
| Dynamic Binding Capacity (g/ml) | ≈30 mg human IgG/ml medium |
| Max. Linear Flow Rate (cm/h) | 700 cm/h |
| pH Stability | 3-12 |
| Pressure (MPa) | 10 |
| Cleaning-in-place (CIP) stability | 0.1-0.5 M NaOH |
| Storage buffer | 20 % ethanol |
| Storage temperature | +4-8 °C |