AM-50 Blue resin

EBL AM-50 Blue resin is Cibacron Blue 3G covalently attached to matrix by the triazine coupling method, giving a highly stable medium with minimal nonspecific adsorption. The rigidity of the matrix, together with a high degree of substitution, enables rapid processing of large volumes at production scale. With excellent binding kinetics, AM-50 Blue is suitable for recovery and purification of albumin and interferon. Purification and concentration are achieved in one single step. The swollen medium has a Cibacron Blue content of > 20 mM dye/mL drained medium. The total binding capacity for human serum albumin is > 18 mg/ml drained medium.

Specifications:
Particle Size (μm) 50
Pore Size (Å) 800
Ligand Density > 20 nM Cibacron Blue 3G/mL
Target Protein Binding Capacity (g/ml) ≥ 18 mg human serum albumin
Bead Matrix Polyacrylate
Recommended Flow Rate (cm/h) 150-700
pH Stability 4-12
Pressure (MPa) 10
Chemical stability 40°C for 7 days in:
70% ethanol, 6 M guanidine Hydrochloride, 8 M urea
Storage buffer 20% ethanol
Storage temperature +4–30 °C
※        EBL AM-50 Blue has high thermal stability and is autoclavable at 121°C for 20 min.

Ordering information:

Catalog Number: amm-5003020, 20 ml

Catalog Number: amm-5003100, 100 ml