EBL RNA Extraction Reagent - is a fast, simple way of preparing RNA for RT-PCR, northern blots, RNase protection assays, and other molecular experiments requiring RNA. EBL RNA Extraction Reagent includes phenol and guanidine thiocyanate which effectively inhibits the RNase activity, while facilitates the isolation of a variety of RNA species of large or small molecular size. Following the addition of chloroform and subsequent centrifugation, the homogenate separates into an aqueous phase, an interphase and an organic phase. RNA is recovered in the aqueous phase with the addition of isopropyl alcohol, and is subsequently washed in ethanol and dissolved in RNase-free water. An optional DNase I treatment may improve certain downstream applications.
Applications:
Preparation of total RNA from a variety samples for RT-PCR, northern blots, and other molecular experiments requiring RNA.
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